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2.9 Immunofluorescence staining of lung tissue After C57BL/6 mice were induced with LPS and papain via intranasal administration, the mice were perfused with 4% PFA, and lung tissue was collected, fixed in 4% PFA (diluted with PBS at a ratio of 1:2) at 4 for 24 h, dehydrated with 30% sucrose, and then prepared as 20-m slices after OCT embedding

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